Particle tracking by repetitive phase-shift interferometric super resolution microscopy
نویسندگان
چکیده
منابع مشابه
Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure.
Understanding molecular-scale architecture of cells requires determination of 3D locations of specific proteins with accuracy matching their nanometer-length scale. Existing electron and light microscopy techniques are limited either in molecular specificity or resolution. Here, we introduce interferometric photoactivated localization microscopy (iPALM), the combination of photoactivated locali...
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Light microscopy techniques have been vital to our understanding of biological structures and systems since their invention in the late 16 Century. However, conventional light microscopy techniques are limited by the diffraction limit of light, preventing us from seeing anything smaller than approximately 200 nm in x and y and approximately 500 nm in z. Many molecules and structures of interest...
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Rayleigh-Sommerfeld back-propagation can be used to reconstruct the three-dimensional light field responsible for the recorded intensity in an in-line hologram. Deconvolving the volumetric reconstruction with an optimal kernel derived from the Rayleigh-Sommerfeld propagator itself emphasizes the objects responsible for the scattering pattern while suppressing both the propagating light and also...
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ژورنال
عنوان ژورنال: Optics Letters
سال: 2018
ISSN: 0146-9592,1539-4794
DOI: 10.1364/ol.43.002819